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Florida's LGBTQ history begins thousands of years before Europeans arrived. Before colonization, most Native American societies in North America had gender roles for people of a mixed or third gender - people known today as two-spirits. Among the earliest existing sources for two-spirits come from the Timucua people of North Florida, who first encountered Europeans in the 16th century.
Read more: https://www.thejaxsonmag.com/article/florida-lgbtq-history-timucua-two-spirits/